Femtosecond Fluorescence Upconversion & TCSPC Extension

Harpia-TF - Light Conversion Ltd.

HARPIA‑TF is a time-resolved fluorescence measurement extension to the HARPIA‑TA mainframe that combines two time-resolved fluorescence techniques. For the highest time resolution, fluorescence is measured using the time-resolved fluorescence upconversion technique, where the fluorescence light emitted from the sample is sum-frequency mixed in a nonlinear crystal with a femtosecond gating pulse from the laser. The time resolution is then limited by the duration of the gate pulse and is in the range of 250 fs. For fluorescence decay times exceeding 150 ps, the instrument can be used in time-correlated single-photon counting (TCSPC) mode that allows for measuring high‑accuracy kinetic traces in the 200 ps – 2 μs temporal domain. HARPIA‑TF extension is designed around the industry leading Becker&Hickl® time-correlated single-photon counting system, with different detector options available.